AccueilEmplois & formationsChercheur postdoctoral, Characterization of the role of Viable But Non Culturable and persister cells in the resistance of Cryptococcus neoformans to antifungal drugs, Institut Pasteur Paris, CDD 36 mois

Chercheur postdoctoral, Characterization of the role of Viable But Non Culturable and persister cells in the resistance of Cryptococcus neoformans to antifungal drugs, Institut Pasteur Paris, CDD 36 mois

Détails de l'offre

  • Type de poste: CDD, Post-doctorat
  • Secteur : Public
  • Localité : France 
  • Limite de candidature : 01/10/2021
  • Profil de poste:
    Recherche et innovation
  • Domaine(s) :
    Microbiologie et Immunologie

Description

Lieu de travail :  Paris – Ile-de-France – France
Champ scientifique principal : Biologie
Champs scientifiques secondaires : Santé, médecine humaine, vétérinaire
Mots clés : résistance, champignons, persisters cells, dormance, microscopie, sorting, épigénétique
Date limite de candidature : 01/10/2021
Fonction : Recherche et Développement

Employeur

L’Institut Pasteur développe de nombreux grands projets internationaux en partenariat avec les grandes instances scientifiques internationales comme l’Organisation mondiale de la santé, et de nombreuses organisations, fondations, instituts de recherche, universités ou autres acteurs privés du monde entier. *

Par ailleurs, l’Institut Pasteur est membre du Pasteur Network, réseau de coopération internationale en santé publique, enseignement et recherche, qui regroupe 33 membres dans le monde.

Poste et missions

Characterization of the role of Viable But Non Culturable and persister cells in the resistance of Cryptococcus neoformans to antifungal drugs

C. neoformans and cryptococcosis are used as organism and disease models for studying dormancy and latency. Indeed, we know that in specific conditions, subpopulation of yeasts harboring various metabolic properties and phenotypes can be generated.

Using drastic conditions (host, macrophage, hypoxia/nutrient deprivation), we know that viable but non culturable cells (VBNC)and stress persister cells are generated.
The definitions of these phenotypes are not fully defined in fungi and deserves much more careful attention to really understand the essence of these different phenotypes.
As a new approach in explaining the appearance of stress and drug resistance in Fungi, I want to explore the link between VBNC, stress persister cells and antifungal drug resistance/tolerance in C. neoformans.
I hypothesize that subpopulations of yeasts could be/become resistant upon exposure to various stresses. These populations can be characterized as either intrinsically tolerant to stress/drugs (VBNC) or associated with acquired resistance upon stress/antifungal drugs exposure (persister cells).
I thus aim at understanding the biological differences of stress and antifungal persister cells subpopulations focusing on metabolism, proteome, transcriptome as compared to bulk populations of and VBNC with the goal to understand how subpopulations adapt and resist to drastic environmental changes and to describe the molecular mechanisms associated with those phenotypic changes in order to explore new drugs and pathways that play a role in controlling tolerance/resistance.

Mobilité géographique :

Pas de déplacement

Prise de fonction :

01/10/2021

Profil

Fluorescence and fluorescence microscopy, Flow cytometry and sorting, KO mutant screening, Molecular biology, Analysis of transcriptome data

Objectifs

3 work package a réaliser en 3 ans.

Encadrement de Master 2

Pour postuler

Candidater sur le site de l’Association Bernard Gregory.